HomeVideoJun Cao |Hangzhou Normal University| China| Mass Spectrometry 2015 | Conference Series LLC 

Jun Cao |Hangzhou Normal University| China| Mass Spectrometry 2015 | Conference Series LLC 



International Summit on Current Trends in Mass Spectrometry July 13-15, 2015 New Orleans, USA

Scientific Talk On:Quantitative analysis of the mitochondrial proteome and phosphoproteome in the yeast Saccharomyces cerevisiae
Biography:
Jun cao is associate professor in Hangzhou Normal University, China. He has done work for a rapid, simple, optimized, and validated miniaturized SPE method for the separation and quantification of six flavanones in Citrus fruit extracts by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry using a dual electrospray ionization source. He has published more than 50 papers.
Abstract:
Traditional solid phase extraction (SPE) method requires large volumes of elution solvent and high consumption of sorbents, making the whole procedure expensive and time consuming. Therefore, to establish a reliable miniaturized SPE approach for the extraction of tested compounds from complex samples is quite meaningful. Moreover, mesoporous molecular sieves have not been explored extensively as a sorbent for miniaturized SPE. In particular, its applications for the extraction of trace compounds from complex fruits are scarce, barring work from our group. This article reported a rapid, simple, optimized, and validated miniaturized SPE method for the separation and quantification of six flavanones in Citrus fruit extracts by ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry using a dual electrospray ionization source. The mesoporous molecular sieve SBA-15 as a novel solid sorbent for the extraction of target analytes was characterized by Fourier transform-infrared spectroscopy and scanning electron microscopy. Additionally, compared with other reported extraction techniques, the mesoporous SBA-15 based SPE method possessed the advantages of shorter analysis time and higher sensitivity. Furthermore, considering the different nature of the tested compounds, all of the parameters, including the SBA-15 amount, solution pH, elution solvent, and the sorbent type, were investigated in detail. Under the optimum condition, the instrumental detection and quantitation limits calculated were less than 4.26 and 14.29 ng mL-1, respectively. The recoveries obtained for all the analytes were ranging from 89.22% to 103.46%. The experimental results suggest that SBA-15 is a promising material for the purification and enrichment of target flavanones from complex citrus fruit samples.
Talk Address:http://massspectra.com/abstract/2015/miniaturized-solid-phase-extraction-of-flavanones-from-citrus-fruits-by-ultrahigh-performance-liquid-chromatography-coupled-with-quadrupole-time-of-flight-tandem-mass-spectrometry
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